Dietary bioactive compounds from natural sources (e.g. herbal medicines, foods) are known to poten-26 tially suppress acute or chronic inflammation and promote the effectiveness of treatment to reduce the harm-27 ful effects of gastritis alone or in combination. In this regard, we have characterized four Cameroonian spice 28 extracts namely Aframomum citratum, Dichrostachys glomerata, Tetrapleura tetraptera, and Xylopia parviflora 29 through reverse phase-high-performance liquid chromatography (RP-HPLC), ultra-performance liquid chro-30 matography-electrospray ionization high-resolution mass spectrometry (UPLC-ESI-HRMS/MS), Fourier 31 transform infrared spectroscopic (FTIR) analyses and investigated their antioxidant and synergistic anti-in-32 flammatory activities in gastric epithelial cells (AGS and GES-1 cells). The extracts showed a high amount of 33 total phenolic (TPC: 150-290 mg GAE/g of extract) and flavonoid content (TFC: 35-115 mg CE/g of extract) 34 with antioxidant properties in a cell-free system (1,1-Diphenyl-2-picryl-hydrazyl (DPPH) IC50s ≤ 45 µg/mL; 35 2,2’-azinobis-(3 ethylbenzothiazoline-6-sulfonic acid) (ABTS) IC50s ≤ 29 µg/mL). The extracts in combination 36 (MIX) exert a synergistic beneficial effect (Combination index (CIs)<1 and Dose reduction index (DRIs)>1) on 37 inflammatory markers (Interleukin (IL)-8 and -6 release, and NF-κB driven transcription) in human gastric 38 epithelial cells, which may result from the presence of phenolic compounds (phenolic acids, flavonoids) or 39 other compounds (protein, lipid, aromatic, polysaccharide compounds) tentatively identified in the extracts. 40 The general findings of the present study provide supporting evidence on the chemical composition of four 41 Cameroonian dietary plants and their significant synergistic inhibitory activities on inflammatory markers of 42 gastric epithelial cells.
Comments