Metabolic syndrome is very complex and is associated with a series of pathologies namely glucose intolerance, diabetes, high blood pressure, dyslipidemia, micro albuminuria, overweight and obesity. The overall prevalence of metabolic syndrome is constantly increasing worldwide and the
initial management of this physiopathology involves lifestyle modifications, including changes in diet and exercise habits. Besides, rather than conventional drugs like Orlistat, herbs/spices that were traditionally used to remedy these diseases have been re-evaluated systematically.The present work chemically characterizes and investigates the in vivo beneficial effects of hydroalcoholic extracts of two Cameroonian spices, focusing on Obesity-related lipid parameters in high
fat-fed C57BL/6 mice. Hydro-ethanolic extracts were prepared and characterized by RP-HPLC-PDA and UPLC-Triple
TOF-ESI-MS/MS analysis. They were orally administrated for 30 days in different doses (200 mg.kg-1 B.W and 100 mg.kg-1 B.W) to obese C57BL/6 mice. Food intake and body weight were recorded every day. Anthropometric, plasma biochemical parameters and lipid content were
estimated at the beginning and end of the experiment. Epididymal and inguinal adipose tissues as well as liver tissue were subjected to histological examinations. Lipid content estimation and FAME analysis were performed in the faecal, liver and adipose tissue samples.Oral administration of the extracts at 200 mg.kg-1 B.W significantly reduced food intake, body
weight. Decreased in liver and white adipose tissue (WAT) weight as well as lipid content in plasma were observed. Plasma enzyme (SGOT, SGPT, ALP) estimation showed there was no damage
to vital organs. The chemical analysis suggested that 4-Caffeoylquinic acid, p Coumaric acid 4-O-glucoside, 5-Caffeoylshikimic acid, 4-O-Methyl gallic acid, Morusin derivatives, Naringenin-7-
O-glucoside, Caffeic acid hexose and 5,3,4’-trihydroxy-7-methoxy-8-methyl homoisoflavanone
identified in extracts could potentially justify the biological properties observed.
Results obtained from this study showed Xylopia parviflora (A. Rich.) Benth and Aframomum citratum (Pereira ex Oliv. et Hanb.) K. Shum decreased lipid accumulation in high‐fat‐diet-induced
obese C57BL/6 mice and confirmed, at least in part, our previous in-vitro and ex-vivo analysis. However, deduction of molecular mechanism of these extracts still need to be explore.
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